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KMID : 1025520000420050649
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Journal of Animal Science and Technology
2000 Volume.42 No. 5 p.649 ~ p.658
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Effect of Grass Lipids and Saponified Grass Lipids on Fermentation Characteristics and Microbial Growth in vitro
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Fujita, Hiroshi
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Abstract
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To investigate the effect of grass lipids as ether extracts from orchardgrass hay on nutrient digestion, fermentation and microbial growth in the rumen, an in vitro incubation trial was carried out using residues of orchardgrass hay from which lipid fraction was removed by ether extraction. Two and a half grams(2.5g) of samples added with 3 and 6% grass lipids and saponified lipids were incubated anaerobically at 39℃ for 12 and 24 h. A mixture of artificial saliva (100㎖) and rumen fluid (100㎖) collected from the rumen of fistulated wethers was used for the incubation trial. Dry matter (DM) disappearance decreased when 6% lipids was added as compared to control. The tendency of decrease in DM disappearance did not recover by the addition of saponified lipids. Disappearance of neutral detergent fiver (NDF) decreased with lipids at 6% level. The addition of 3% saponified lipids showed a tendency to improve NDF disappearance, but not with 6% level. Microbial nitrogen (N) was not affected by the addition of lipids after incubation, except decreased at 12 h when 6% saponified lipids was added. However, microbial N per DM disappeared increased in both treatments with 6% grass lipids and saponified lipids irrespective of the incubation time at 12 and 24 h. Addition of lipids and saponified lipids gave no effect on ammoniacal and water-soluble N, and total volatile fatty acids and its molar proportion among treatments, except increased to ammoniacal N at 12 h when 6% was added.
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